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p53 dependent fluc reporter plasmid pg13 luc  (Addgene inc)


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    Structured Review

    Addgene inc p53 dependent fluc reporter plasmid pg13 luc
    tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for <t>p53</t> and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.
    P53 Dependent Fluc Reporter Plasmid Pg13 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p53+dependent+fluc+reporter+plasmid+pg13+luc/PG13-Luc+(p53)+(Plasmid+%2316251)/pmc12204705-163-21-31
    Average 93 stars, based on 53 article reviews
    p53 dependent fluc reporter plasmid pg13 luc - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations"

    Article Title: An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf532

    tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.
    Figure Legend Snippet: tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.

    Techniques Used: Western Blot, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay

    Related Articles

    Transfection:

    Article Title: An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations
    Article Snippet: .. The following day, cells were transfected with 50 ng of the indicated sup-tRNA plasmid (or an empty vector), 50 ng of p53-dependent Fluc reporter plasmid PG13-luc (a gift from Bert Vogelstein; Addgene Plasmid # 16442; http://n2t.net/addgene:16442; RRID:ADDGENE_16442 ), and 15 ng of pLX313- Renilla . .. A549 and SK-MES-1 cells were transfected with FuGENE ® 6 Transfection Reagent (Promega), whereas C2BBe1 cells were transfected with PEI-MAX (Kyfora Bio).

    Plasmid Preparation:

    Article Title: An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations
    Article Snippet: .. The following day, cells were transfected with 50 ng of the indicated sup-tRNA plasmid (or an empty vector), 50 ng of p53-dependent Fluc reporter plasmid PG13-luc (a gift from Bert Vogelstein; Addgene Plasmid # 16442; http://n2t.net/addgene:16442; RRID:ADDGENE_16442 ), and 15 ng of pLX313- Renilla . .. A549 and SK-MES-1 cells were transfected with FuGENE ® 6 Transfection Reagent (Promega), whereas C2BBe1 cells were transfected with PEI-MAX (Kyfora Bio).



    Similar Products

    p53 dependent fluc reporter plasmid pg13 luc  (Addgene inc)
    93
    Addgene inc p53 dependent fluc reporter plasmid pg13 luc
    tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for <t>p53</t> and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.
    P53 Dependent Fluc Reporter Plasmid Pg13 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p53+dependent+fluc+reporter+plasmid+pg13+luc/PG13-Luc+(p53)+(Plasmid+%2316251)/pmc12204705-163-21-31
    Average 93 stars, based on 1 article reviews
    p53 dependent fluc reporter plasmid pg13 luc - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.

    Journal: Nucleic Acids Research

    Article Title: An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations

    doi: 10.1093/nar/gkaf532

    Figure Lengend Snippet: tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.

    Article Snippet: The following day, cells were transfected with 50 ng of the indicated sup-tRNA plasmid (or an empty vector), 50 ng of p53-dependent Fluc reporter plasmid PG13-luc (a gift from Bert Vogelstein; Addgene Plasmid # 16442; http://n2t.net/addgene:16442; RRID:ADDGENE_16442 ), and 15 ng of pLX313- Renilla .

    Techniques: Western Blot, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay